Experiments can be divided into fluid proteomics and tissue proteomics. Fluid proteomics involves the characterization of proteins in body fluids, such as serum or CSF.

Proteomics

Experiments can be divided into fluid proteomics and tissue proteomics. Fluid proteomics involves the characterization of proteins in body fluids, such as serum or CSF. Tissue proteomics involves Biochemical purification strategies to enrich proteins from cellular fractions and is often used as a first step in the identification and quantification. Protein isolation includes multi-step gradient centrifugation. The tissue proteomics experiments typically involve the identification and quantification of large sets of proteins in neuronal tissues under different conditions.

Proteomics experiments are generally complex in their set-up and data analysis. Therefore, expert advice on the set-up of the multi-day proteomics experiments is available and will generally be required for the proper execution of the experiments. Full service will involve a fully executed proteomics experiment in which the sample(s) is (are) the startng point of the experiment. Customized service includes a subset of experiments, for instance, only mass spectrometry of the sample.

Two-dimensional gel electrophoresis
Proteomics might include two-dimensional gelelectrophoresis in 12 gel Bio-rad systems for the separation of proteins on isoelectric point and mass. The technique is suited for the identification of posttranslational events and protein identification from gel using post-gel mass spectrometric analysis. Also, quantification of proteins on 6 versus 6 gels is possible). Full service and customized services are possible.

Liquid chromatography and mass spectrometry
In addition, a proteomics pipeline consisting of two-dimensional liquid chromatography (anion exchange and nano-LC system), a spotting robot for nano-LC fractions and subsequent MALDI-TOF mass spectrometry (ABI-4800 platform) is available. This workflow allows identification of proteins and quantification using isobaric labeling using a customized database system and protein data analysis across experiments. Typically hundreds of proteins can be identified and quantified in a single experiment. Using novel labeling techniques it is possible to differentially quantify proteins with excellent statstical power. Full service is possible.